Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

This protocol describes a method developed to identify endogenous peptides in human cerebrospinal fluid (CSF). For this purpose, a previously developed method based on molecular weight cut-off (MWCO) filtration and mass spectrometric analysis was combined with an offline high-pH reverse phase HPLC p...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Hansson, Karl T., Skillbäck, Tobias, Pernevik, Elin, Holmén-Larsson, Jessica, Brinkmalm, Gunnar, Blennow, Kaj, Zetterberg, Henrik, Gobom, Johan
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2017
Teme:
Neuroscience
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755527/
https://ncbi.nlm.nih.gov/pubmed/29286401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56244
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