A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells

Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5′-TTTN-3′ protospacer adjacent motif (PAM) at the 5′ end of the protospacer for genome editing. For widesp...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Nucleic Acids Res
Hlavní autoři: Tu, Mengjun, Lin, Li, Cheng, Yilu, He, Xiubin, Sun, Huihui, Xie, Haihua, Fu, Junhao, Liu, Changbao, Li, Jin, Chen, Ding, Xi, Haitao, Xue, Dongyu, Liu, Qi, Zhao, Junzhao, Gao, Caixia, Song, Zongming, Qu, Jia, Gu, Feng
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2017
Témata:
Nucleic Acid Enzymes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737432/
https://ncbi.nlm.nih.gov/pubmed/28977650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx783
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky