A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells

Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5′-TTTN-3′ protospacer adjacent motif (PAM) at the 5′ end of the protospacer for genome editing. For widesp...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Tu, Mengjun, Lin, Li, Cheng, Yilu, He, Xiubin, Sun, Huihui, Xie, Haihua, Fu, Junhao, Liu, Changbao, Li, Jin, Chen, Ding, Xi, Haitao, Xue, Dongyu, Liu, Qi, Zhao, Junzhao, Gao, Caixia, Song, Zongming, Qu, Jia, Gu, Feng
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2017
Soggetti:
Nucleic Acid Enzymes
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737432/
https://ncbi.nlm.nih.gov/pubmed/28977650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx783
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