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Optimization of CRISPR-Cas9 genome editing for loss-of-function in the early chick embryo
The advent of CRISPR/Cas9 has made genome editing possible in virtually any organism, including those not previously amenable to genetic manipulations. Here, we present an optimization of CRISPR/Cas9 for application to early avian embryos with improved efficiency via a three-fold strategy. First, we...
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| Udgivet i: | Dev Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5728388/ https://ncbi.nlm.nih.gov/pubmed/29150011 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ydbio.2017.08.036 |
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