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Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9
The CRISPR/Cas9 system has ushered in a new era of targeted genetic manipulations. Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirt Ciona intestinalis. We use electroporation to deliver CRISPR/Cas9 components for tissue-specific disruption of th...
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| Publicado no: | Development |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Company of Biologists
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4302896/ https://ncbi.nlm.nih.gov/pubmed/25336740 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.114488 |
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