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CRISPR knockouts in Ciona embryos
CRISPR/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9...
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| Udgivet i: | Adv Exp Med Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2018
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6061950/ https://ncbi.nlm.nih.gov/pubmed/29542087 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-981-10-7545-2_13 |
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