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A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos

An important strategy for establishing mechanisms of gene function during development is through mutation of individual genes and analysis of subsequent effects on cell behavior. Here, we present a single-plasmid approach for genome editing in chick embryos to study experimentally perturbed cells in...

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Detalhes bibliográficos
Publicado no:Development
Main Authors: Gandhi, Shashank, Li, Yuwei, Tang, Weiyi, Christensen, Jens B., Urrutia, Hugo A., Vieceli, Felipe M., Piacentino, Michael L., Bronner, Marianne E.
Formato: Artigo
Idioma:Inglês
Publicado em: The Company of Biologists Ltd 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8077534/
https://ncbi.nlm.nih.gov/pubmed/33688075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.193565
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