Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation

The generation of gene-edited animals using the CRISPRs/Cas9 system is based on microinjection into zygotes which is inefficient, time consuming and demands high technical skills. We report the optimization of an electroporation method for intact rat zygotes using sgRNAs and Cas9 protein in combinat...

Full description

Saved in:
Bibliographic Details
Published in:Sci Rep
Main Authors: Remy, Séverine, Chenouard, Vanessa, Tesson, Laurent, Usal, Claire, Ménoret, Séverine, Brusselle, Lucas, Heslan, Jean-Marie, Nguyen, Tuan Huan, Bellien, Jeremy, Merot, Jean, De Cian, Anne, Giovannangeli, Carine, Concordet, Jean-Paul, Anegon, Ignacio
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2017
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5707420/
https://ncbi.nlm.nih.gov/pubmed/29185448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16328-y
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items