Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation

The generation of gene-edited animals using the CRISPRs/Cas9 system is based on microinjection into zygotes which is inefficient, time consuming and demands high technical skills. We report the optimization of an electroporation method for intact rat zygotes using sgRNAs and Cas9 protein in combinat...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Remy, Séverine, Chenouard, Vanessa, Tesson, Laurent, Usal, Claire, Ménoret, Séverine, Brusselle, Lucas, Heslan, Jean-Marie, Nguyen, Tuan Huan, Bellien, Jeremy, Merot, Jean, De Cian, Anne, Giovannangeli, Carine, Concordet, Jean-Paul, Anegon, Ignacio
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5707420/
https://ncbi.nlm.nih.gov/pubmed/29185448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16328-y
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