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Disassembly/Reassembly Strategy for the Production of Highly Pure GroEL, a Tetradecameric Supramolecular Machine, Suitable for Quantitative NMR, EPR and Mutational Studies
GroEL, a prototypical member of the chaperonin class of chaperones, is a large supramocular machine that assists protein folding and plays an important role in proteostasis. GroEL comprises two heptameric rings, each of which encloses a large cavity that provides a folding chamber for protein substr...
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| Опубликовано в: : | Protein Expr Purif |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5669812/ https://ncbi.nlm.nih.gov/pubmed/28951283 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.09.010 |
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