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Long distance measurements up to 160 Å in the GroEL tetradecamer using Q-band DEER EPR spectroscopy

Current distance measurements between spin-labels on multimeric protonated proteins using double electron-electron resonance (DEER) EPR spectroscopy are generally limited to the 15–60 Å range. Here we show how DEER experiments can be extended to dipolar evolution times of ~80 µs, permitting distance...

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發表在:Angew Chem Int Ed Engl
Main Authors: Schmidt, Thomas, Wälti, Marielle A., Baber, James L., Hustedt, Eric J., Clore, G. Marius
格式: Artigo
語言:Inglês
出版: 2016
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5166617/
https://ncbi.nlm.nih.gov/pubmed/27860003
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201609617
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