Načítá se...
Protocol for Construction of a Tunable CRISPR Interference (tCRISPRi) Strain for Escherichia coli
We present a protocol for construction of tunable CRISPR interference (tCRISPRi) strains for Escherichia coli. The tCRISPRi system alleviates most of the known problems of plasmid-based expression methods, and can be immediately used to construct libraries of sgRNAs that can complement the Keio coll...
Uloženo v:
| Vydáno v: | Bio Protoc |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-Protocol
2017
|
| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5659595/ https://ncbi.nlm.nih.gov/pubmed/29082292 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2574 |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!
|