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Protocol for Construction of a Tunable CRISPR Interference (tCRISPRi) Strain for Escherichia coli
We present a protocol for construction of tunable CRISPR interference (tCRISPRi) strains for Escherichia coli. The tCRISPRi system alleviates most of the known problems of plasmid-based expression methods, and can be immediately used to construct libraries of sgRNAs that can complement the Keio coll...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5659595/ https://ncbi.nlm.nih.gov/pubmed/29082292 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2574 |
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