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Fundamental CRISPR-Cas9 tools and current applications in microbial systems

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Synth Syst Biotechnol
Hauptverfasser: Tian, Pingfang, Wang, Jia, Shen, Xiaolin, Rey, Justin Forrest, Yuan, Qipeng, Yan, Yajun
Format: Artigo
Sprache:Inglês
Veröffentlicht: KeAi Publishing 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5655352/
https://ncbi.nlm.nih.gov/pubmed/29318202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2017.08.006
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