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Fundamental CRISPR-Cas9 tools and current applications in microbial systems

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...

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Dades bibliogràfiques
Publicat a:Synth Syst Biotechnol
Autors principals: Tian, Pingfang, Wang, Jia, Shen, Xiaolin, Rey, Justin Forrest, Yuan, Qipeng, Yan, Yajun
Format: Artigo
Idioma:Inglês
Publicat: KeAi Publishing 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5655352/
https://ncbi.nlm.nih.gov/pubmed/29318202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2017.08.006
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