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Fundamental CRISPR-Cas9 tools and current applications in microbial systems

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...

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Podrobná bibliografie
Vydáno v:Synth Syst Biotechnol
Hlavní autoři: Tian, Pingfang, Wang, Jia, Shen, Xiaolin, Rey, Justin Forrest, Yuan, Qipeng, Yan, Yajun
Médium: Artigo
Jazyk:Inglês
Vydáno: KeAi Publishing 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5655352/
https://ncbi.nlm.nih.gov/pubmed/29318202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2017.08.006
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