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Fundamental CRISPR-Cas9 tools and current applications in microbial systems
Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...
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| Published in: | Synth Syst Biotechnol |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
KeAi Publishing
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5655352/ https://ncbi.nlm.nih.gov/pubmed/29318202 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2017.08.006 |
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