Fundamental CRISPR-Cas9 tools and current applications in microbial systems

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...

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Bibliographic Details
Published in:Synth Syst Biotechnol
Main Authors: Tian, Pingfang, Wang, Jia, Shen, Xiaolin, Rey, Justin Forrest, Yuan, Qipeng, Yan, Yajun
Format: Artigo
Language:Inglês
Published: KeAi Publishing 2017
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5655352/
https://ncbi.nlm.nih.gov/pubmed/29318202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2017.08.006
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