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Purification of FLAG-tagged Secreted Proteins from Mammalian Cells
This protocol describes a method for purifying glycosylated FLAG-tagged secreted proteins with disulfide bonds from mammalian cells. The purified products can be used for various applications, such as ligand binding assays.
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| Publicado no: | Bio Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5654507/ https://ncbi.nlm.nih.gov/pubmed/29075655 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2430 |
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