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An improved strategy for fluorescent tagging of membrane proteins for overexpression and purification in mammalian cells
An essential prerequisite for in vitro biochemical or structural studies is a construct that is amenable to high level expression and purification and is biochemically “well-behaved”. In the field of membrane protein research, the use of Green Fluorescent Protein (GFP) to monitor and optimize the he...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7266526/ https://ncbi.nlm.nih.gov/pubmed/30481009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b01070 |
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