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Immunoaffinity Purification of Protein Complexes from Mammalian Cells

In this chapter, we describe a purification scheme designed to isolate multisubunit protein complexes gently and quickly from crude extracts of mammalian cells using immunoaffinity purification of epitope tagged proteins and the multisubunit complexes with which they associate. As an example we desc...

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Détails bibliographiques
Auteurs principaux: Tomomori-Sato, Chieri, Sato, Shigeo, Conaway, Ronald C., Conaway, Joan W.
Format: Artigo
Langue:Inglês
Publié: 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3693849/
https://ncbi.nlm.nih.gov/pubmed/23436370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-284-1_22
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