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Immunoaffinity Purification of Protein Complexes from Mammalian Cells
In this chapter, we describe a purification scheme designed to isolate multisubunit protein complexes gently and quickly from crude extracts of mammalian cells using immunoaffinity purification of epitope tagged proteins and the multisubunit complexes with which they associate. As an example we desc...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3693849/ https://ncbi.nlm.nih.gov/pubmed/23436370 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-284-1_22 |
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