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Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding
Green fluorescent protein (GFP) variants are widely used as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering their structure to develop in vivo optical sensors, such as for optogenetics and force transduction. Ensemble experiments have shown that the fl...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2017
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5651744/ https://ncbi.nlm.nih.gov/pubmed/29073015 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1704937114 |
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