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Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Green fluorescent protein (GFP) variants are widely used as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering their structure to develop in vivo optical sensors, such as for optogenetics and force transduction. Ensemble experiments have shown that the fl...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Ganim, Ziad, Rief, Matthias
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5651744/
https://ncbi.nlm.nih.gov/pubmed/29073015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1704937114
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