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Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Green fluorescent protein (GFP) variants are widely used as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering their structure to develop in vivo optical sensors, such as for optogenetics and force transduction. Ensemble experiments have shown that the fl...

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Bibliografiska uppgifter
I publikationen:Proc Natl Acad Sci U S A
Huvudupphovsmän: Ganim, Ziad, Rief, Matthias
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2017
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5651744/
https://ncbi.nlm.nih.gov/pubmed/29073015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1704937114
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