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Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Green fluorescent protein (GFP) variants are widely used as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering their structure to develop in vivo optical sensors, such as for optogenetics and force transduction. Ensemble experiments have shown that the fl...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Ganim, Ziad, Rief, Matthias
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5651744/
https://ncbi.nlm.nih.gov/pubmed/29073015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1704937114
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