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Tracking Unfolding and Refolding of Single GFPmut2 Molecules

The unfolding and refolding kinetics of >600 single GFPmut2 molecules, entrapped in wet nanoporous silica gels, were followed by monitoring simultaneously the fluorescence emission of the anionic and neutral state of the chromophore, primed by two-photon excitation. The rate of unfolding, induced...

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Bibliografski detalji
Glavni autori: Cannone, Fabio, Bologna, Sara, Campanini, Barbara, Diaspro, Alberto, Bettati, Stefano, Mozzarelli, Andrea, Chirico, Giuseppe
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366706/
https://ncbi.nlm.nih.gov/pubmed/15994904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.064584
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