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Two-Photon Thermal Bleaching of Single Fluorescent Molecules

We have studied the fluorescence emission by two-photon excitation of four dyes widely used for bioimaging studies, rhodamine 6G, fluorescein, pyrene and indo-1 at the single molecule level. The single dye molecules, spread on a glass substrate by spin coating, show a constant fluorescence output un...

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Detalles Bibliográficos
Autores principales: Chirico, Giuseppe, Cannone, Fabio, Baldini, Giancarlo, Diaspro, Alberto
Formato: Artigo
Lenguaje:Inglês
Publicado: Biophysical Society 2003
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302640/
https://ncbi.nlm.nih.gov/pubmed/12524312
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