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Evidence of Discrete Substates and Unfolding Pathways in Green Fluorescent Protein

We present evidence of conformational substates of a green fluorescent protein mutant, GFPmut2, and of their relationship with the protein behavior during chemical unfolding. The fluorescence of single molecules, excited by two infrared photons from a pulsed laser, was detected in two separate chann...

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Bibliografski detalji
Glavni autori: Baldini, Giancarlo, Cannone, Fabio, Chirico, Giuseppe, Collini, Maddalena, Campanini, Barbara, Bettati, Stefano, Mozzarelli, Andrea
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2007
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1796838/
https://ncbi.nlm.nih.gov/pubmed/17142282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.093567
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