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Evidence of Discrete Substates and Unfolding Pathways in Green Fluorescent Protein
We present evidence of conformational substates of a green fluorescent protein mutant, GFPmut2, and of their relationship with the protein behavior during chemical unfolding. The fluorescence of single molecules, excited by two infrared photons from a pulsed laser, was detected in two separate chann...
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| Main Authors: | , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Biophysical Society
2007
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1796838/ https://ncbi.nlm.nih.gov/pubmed/17142282 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.093567 |
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