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GFP-mut2 Proteins in Trehalose-Water Matrixes: Spatially Heterogeneous Protein-Water-Sugar Structures

We report investigations on the properties of nanoenvironments around single-GFP-mut2 proteins in trehalose-water matrixes. Single-GFPmut2 molecules embedded in thin trehalose-water films were characterized in terms of their fluorescence brightness, bleaching dynamics, excited state lifetime, and fl...

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Hlavní autoři: D'Alfonso, Laura, Collini, Maddalena, Cannone, Fabio, Chirico, Giuseppe, Campanini, Barbara, Cottone, Grazia, Cordone, Lorenzo
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2007
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1914445/
https://ncbi.nlm.nih.gov/pubmed/17416616
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.090621
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