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GFP-mut2 Proteins in Trehalose-Water Matrixes: Spatially Heterogeneous Protein-Water-Sugar Structures

We report investigations on the properties of nanoenvironments around single-GFP-mut2 proteins in trehalose-water matrixes. Single-GFPmut2 molecules embedded in thin trehalose-water films were characterized in terms of their fluorescence brightness, bleaching dynamics, excited state lifetime, and fl...

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Detalhes bibliográficos
Main Authors: D'Alfonso, Laura, Collini, Maddalena, Cannone, Fabio, Chirico, Giuseppe, Campanini, Barbara, Cottone, Grazia, Cordone, Lorenzo
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1914445/
https://ncbi.nlm.nih.gov/pubmed/17416616
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.090621
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