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High-Throughput Screening of HECT E3 Ubiquitin Ligases Using UbFluor

HECT E3 ubiquitin ligases are responsible for many human disease phenotypes, and are promising drug targets. However, screening assays for HECT E3 inhibitors are inherently complex, requiring upstream E1 and E2 enzymes as well as ubiquitin, ATP, and detection reagents. Intermediate ubiquitin thioest...

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Veröffentlicht in:Curr Protoc Chem Biol
Hauptverfasser: Foote, Peter K., Krist, David T., Statsyuk, Alexander V.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5650195/
https://ncbi.nlm.nih.gov/pubmed/28910856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.24
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