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High-Throughput Screening of HECT E3 Ubiquitin Ligases Using UbFluor
HECT E3 ubiquitin ligases are responsible for many human disease phenotypes, and are promising drug targets. However, screening assays for HECT E3 inhibitors are inherently complex, requiring upstream E1 and E2 enzymes as well as ubiquitin, ATP, and detection reagents. Intermediate ubiquitin thioest...
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| Publicado en: | Curr Protoc Chem Biol |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5650195/ https://ncbi.nlm.nih.gov/pubmed/28910856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.24 |
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