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UbFluor: A Fluorescent Thioester to Monitor HECT E3 Ligase Catalysis
HECT E3 ubiquitin ligases (~28 known) are associated with many phenotypes in eukaryotes, and are important drug targets. However, assays used to screen for small molecule inhibitors of HECT E3s are complex and require ATP, Ub, E1, E2, and HECT E3 enzymes, producing three covalent thioester enzyme in...
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| Publicado no: | Curr Protoc Chem Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5335898/ https://ncbi.nlm.nih.gov/pubmed/28253433 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.17 |
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