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Interpreting reverse transcriptase termination and mutation events for greater insight into the chemical probing of RNA

Chemical probing has the power to provide insight into RNA conformation in vivo and in vitro, but interpreting the results depends on methods to detect the chemically modified nucleotides. Traditionally, the presence of modified bases was inferred from their ability to halt reverse transcriptase dur...

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Detalles Bibliográficos
Publicado en:Biochemistry
Main Authors: Sexton, Alec N., Wang, Peter Y., Rutenberg-Schoenberg, Michael, Simon, Matthew D.
Formato: Artigo
Idioma:Inglês
Publicado: 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5648349/
https://ncbi.nlm.nih.gov/pubmed/28820243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00323
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