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Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging

Genetic code expansion and bioorthogonal labeling provide for the first time a way for direct, site-specific labeling of proteins with fluorescent-dyes in live cells. Although the small size and superb photophysical parameters of fluorescent-dyes offer unique advantages for high-resolution microscop...

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Detalhes bibliográficos
Publicado no:Mol Biol Cell
Main Authors: Schvartz, Tomer, Aloush, Noa, Goliand, Inna, Segal, Inbar, Nachmias, Dikla, Arbely, Eyal, Elia, Natalie
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Cell Biology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5638579/
https://ncbi.nlm.nih.gov/pubmed/28835375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E17-03-0161
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