Internally ratiometric fluorescent sensors for evaluation of intracellular GTP levels and distribution

GTP is a major regulator of multiple cellular processes, but tools for quantitative evaluation of GTP levels in live cells have not been available. Here we report characterization of genetically encoded GTP sensors, constructed by inserting cpYFP into a region of the bacterial FeoB G-protein that un...

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Veröffentlicht in:Nat Methods
Hauptverfasser: Bianchi-Smiraglia, Anna, Rana, Mitra S., Foley, Colleen E., Paul, Leslie M., Lipchick, Brittany C., Moparthy, Sudha, Moparthy, Kalyana, Fink, Emily E., Bagati, Archis, Hurley, Edward, Affronti, Hayley C., Bakin, Andrei V., Kandel, Eugene S., Smiraglia, Dominic J., Feltri, Maria Laura, Sousa, Rui, Nikiforov, Mikhail A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2017
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5636219/
https://ncbi.nlm.nih.gov/pubmed/28869758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4404
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