CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, includin...

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Publicat a:Nat Chem Biol
Autors principals: Zhang, Mingzi M, Wong, Fong Tian, Wang, Yajie, Luo, Shangwen, Lim, Yee Hwee, Heng, Elena, Yeo, Wan Lin, Cobb, Ryan E, Enghiad, Behnam, Ang, Ee Lui, Zhao, Huimin
Format: Artigo
Idioma:Inglês
Publicat: 2017
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5634907/
https://ncbi.nlm.nih.gov/pubmed/28398287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2341
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