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Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces

CRISPR-Cas9 has proven as a very powerful gene editing tool for Actinomyces, allowing scarless and precise genome editing in selected strains of these biotechnologically relevant microorganisms. However, its general application in actinomycetes has been limited due to its inefficacy when applying th...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Ind Microbiol Biotechnol
Prif Awduron: Ye, Suhui, Enghiad, Behnam, Zhao, Huimin, Takano, Eriko
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Springer International Publishing 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7244461/
https://ncbi.nlm.nih.gov/pubmed/32367443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10295-020-02277-5
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