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Tuning SpyTag–SpyCatcher mutant pairs toward orthogonal reactivity encryption

Genetically encoded covalent peptide tagging technology, such as the SpyTag–SpyCatcher reaction, has emerged as a unique way to do chemistry with proteins. Herein, we report the reactivity engineering of SpyTag–SpyCatcher mutant pairs and show that distinct reactivity can be encrypted for the same r...

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Detalhes bibliográficos
Publicado no:Chem Sci
Main Authors: Liu, Yajie, Liu, Dong, Yang, Wei, Wu, Xia-Ling, Lai, Luhua, Zhang, Wen-Bin
Formato: Artigo
Idioma:Inglês
Publicado em: Royal Society of Chemistry 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5627348/
https://ncbi.nlm.nih.gov/pubmed/28989685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc02686b
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