Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction

The display of complex proteins on the surface of cells is of great importance for protein engineering and other fields of biotechnology. Herein, we describe a modular approach, in which the membrane anchor protein Lpp‐OmpA and a protein of interest (passenger) are expressed independently as genetic...

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Bibliographic Details
Published in:Chembiochem
Main Authors: Gallus, Sabrina, Peschke, Theo, Paulsen, Malte, Burgahn, Teresa, Niemeyer, Christof M., Rabe, Kersten S.
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2020
Subjects:
Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7497234/
https://ncbi.nlm.nih.gov/pubmed/32182402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.202000102
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