ロード中...

SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase

SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type,...

詳細記述

保存先:
書誌詳細
出版年:PLoS One
主要な著者: Si, Meng, Xu, Qing, Jiang, Ling, Huang, He
フォーマット: Artigo
言語:Inglês
出版事項: Public Library of Science 2016
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5033358/
https://ncbi.nlm.nih.gov/pubmed/27658030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0162318
タグ: タグ追加
タグなし, このレコードへの初めてのタグを付けませんか!