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SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase
SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type,...
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| 出版年: | PLoS One |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Public Library of Science
2016
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5033358/ https://ncbi.nlm.nih.gov/pubmed/27658030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0162318 |
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