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SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase
SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type,...
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| Publicat a: | PLoS One |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Public Library of Science
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5033358/ https://ncbi.nlm.nih.gov/pubmed/27658030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0162318 |
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