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SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme

SpyTag is a peptide which spontaneously forms an amide bond to its protein partner SpyCatcher. Here we fused SpyTag at the N-terminus of β-lactamase and SpyCatcher at the C-terminus, so the partners could cyclize to lock together the termini of the enzyme. Wild-type enzyme aggregates above 37 °C, wi...

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Pubblicato in:Angew Chem Int Ed Engl
Autori principali: Schoene, Christopher, Fierer, Jacob O., Bennett, S. Paul, Howarth, Mark
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4286826/
https://ncbi.nlm.nih.gov/pubmed/24817566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201402519
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