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SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme
SpyTag is a peptide which spontaneously forms an amide bond to its protein partner SpyCatcher. Here we fused SpyTag at the N-terminus of β-lactamase and SpyCatcher at the C-terminus, so the partners could cyclize to lock together the termini of the enzyme. Wild-type enzyme aggregates above 37 °C, wi...
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| Publicado en: | Angew Chem Int Ed Engl |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4286826/ https://ncbi.nlm.nih.gov/pubmed/24817566 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201402519 |
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