A carregar...

SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme

SpyTag is a peptide which spontaneously forms an amide bond to its protein partner SpyCatcher. Here we fused SpyTag at the N-terminus of β-lactamase and SpyCatcher at the C-terminus, so the partners could cyclize to lock together the termini of the enzyme. Wild-type enzyme aggregates above 37 °C, wi...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Schoene, Christopher, Fierer, Jacob O., Bennett, S. Paul, Howarth, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4286826/
https://ncbi.nlm.nih.gov/pubmed/24817566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201402519
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!