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Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol

Human PrimPol is a DNA primase/polymerase involved in DNA damage tolerance and prevents nuclear genome instability. PrimPol is also localized to the mitochondria, but its precise function in mitochondrial DNA maintenance has remained elusive. PrimPol works both as a translesion (TLS) polymerase and...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Boldinova, Elizaveta O., Stojkovič, Gorazd, Khairullin, Rafil, Wanrooij, Sjoerd, Makarova, Alena V.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5597260/
https://ncbi.nlm.nih.gov/pubmed/28902865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0184489
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