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Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol

Human PrimPol is a DNA primase/polymerase involved in DNA damage tolerance and prevents nuclear genome instability. PrimPol is also localized to the mitochondria, but its precise function in mitochondrial DNA maintenance has remained elusive. PrimPol works both as a translesion (TLS) polymerase and...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Boldinova, Elizaveta O., Stojkovič, Gorazd, Khairullin, Rafil, Wanrooij, Sjoerd, Makarova, Alena V.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5597260/
https://ncbi.nlm.nih.gov/pubmed/28902865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0184489
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