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Top-down/bottom-up mass spectrometry workflow using dissolvable polyacrylamide gels
Biologists’ preeminent toolbox for separating, analyzing, and visualizing proteins is SDS-PAGE, yet recovering the proteins embedded in these polyacrylamide media as intact species is a long-standing challenge for mass spectrometry. In conventional workflows, protein mixtures from crude biological s...
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| Publicado no: | Anal Chem |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5590889/ https://ncbi.nlm.nih.gov/pubmed/28723075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.7b00357 |
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