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Enhancing Protein Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry

The application of ion pre-activation with 266 nm ultraviolet (UV) laser irradiation combined with electron capture dissociation (ECD) is demonstrated to enhance top-down mass spectrometry sequence coverage of disulfide bond containing proteins. UV-based activation can homolytically cleave a disulfi...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Int J Mass Spectrom
Prif Awduron: Wongkongkathep, Piriya, Li, Huilin, Zhang, Xing, Loo, Rachel R. Ogorzalek, Julian, Ryan R., Loo, Joseph A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2015
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4669582/
https://ncbi.nlm.nih.gov/pubmed/26644781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ijms.2015.07.008
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