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Highly efficient preparation of single-stranded DNA rings by T4 ligase at abnormally low Mg(II) concentration
Preparation of large amount of single-stranded circular DNA in high selectivity is crucial for further developments of nanotechnology and other DNA sciences. Herein, a simple but practically useful methodology to prepare DNA rings has been presented. One of the essential factors is to use highly dil...
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| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5587803/ https://ncbi.nlm.nih.gov/pubmed/28655200 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx553 |
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