Genetic interaction mapping in mammalian cells using CRISPR interference

We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pair...

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Publicat a:Nat Methods
Autors principals: Du, Dan, Roguev, Assen, Gordon, David E, Chen, Meng, Chen, Si-Han, Shales, Michael, Shen, John Paul, Ideker, Trey, Mali, Prashant, Qi, Lei S, Krogan, Nevan J
Format: Artigo
Idioma:Inglês
Publicat: 2017
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5584685/
https://ncbi.nlm.nih.gov/pubmed/28481362
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4286
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