Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions

We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual-guide RNAs in three cell lines, altogether comprising 141,912 tests of interaction. Numerous t...

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Pubblicato in:Nat Methods
Autori principali: Shen, John Paul, Zhao, Dongxin, Sasik, Roman, Luebeck, Jens, Birmingham, Amanda, Bojorquez-Gomez, Ana, Licon, Katherine, Klepper, Kristin, Pekin, Daniel, Beckett, Alex, Sanchez, Kyle, Thomas, Alex, Kuo, Chih-Chung, Du, Dan, Roguev, Assen, Lewis, Nathan E., Chang, Aaron N., Kreisberg, Jason F., Krogan, Nevan, Qi, Lei, Ideker, Trey, Mali, Prashant
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2017
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5449203/
https://ncbi.nlm.nih.gov/pubmed/28319113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4225
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