Structure-Based Design of Supercharged, Highly Thermoresistant Antibodies

Mutation of surface residues to charged amino acids increases resistance to aggregation and can enable reversible unfolding. We have developed a protocol using the Rosetta computational design package that “supercharges” proteins while considering the energetic implications of each mutation. Using a...

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Publicado en:Chem Biol
Main Authors: Miklos, Aleksandr E., Kluwe, Christien, Der, Bryan S., Pai, Supriya, Sircar, Aroop, Hughes, Randall A., Berrondo, Monica, Xu, Jianqing, Codrea, Vlad, Buckley, Patricia E., Calm, Alena M., Welsh, Heather S., Warner, Candice R., Zacharko, Melody A., Carney, James P., Gray, Jeffrey J., Georgiou, George, Kuhlman, Brian, Ellington, Andrew D.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5583727/
https://ncbi.nlm.nih.gov/pubmed/22520751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2012.01.018
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