Structure-Based Design of Supercharged, Highly Thermoresistant Antibodies

Mutation of surface residues to charged amino acids increases resistance to aggregation and can enable reversible unfolding. We have developed a protocol using the Rosetta computational design package that “supercharges” proteins while considering the energetic implications of each mutation. Using a...

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Pubblicato in:Chem Biol
Autori principali: Miklos, Aleksandr E., Kluwe, Christien, Der, Bryan S., Pai, Supriya, Sircar, Aroop, Hughes, Randall A., Berrondo, Monica, Xu, Jianqing, Codrea, Vlad, Buckley, Patricia E., Calm, Alena M., Welsh, Heather S., Warner, Candice R., Zacharko, Melody A., Carney, James P., Gray, Jeffrey J., Georgiou, George, Kuhlman, Brian, Ellington, Andrew D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5583727/
https://ncbi.nlm.nih.gov/pubmed/22520751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2012.01.018
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