Transient and dynamic DNA supercoiling potently stimulates the leu-500 promoter in Escherichia coli

The inactive prokaryotic leu-500 promoter (P(leu-500)) contains a single A-to-G point mutation in the −10 region of the leucine operon promoter, which causes leucine auxotrophy. This promoter can be activated by (−) DNA supercoiling in Escherichia coli topA strains. However, whether this activation...

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Bibliographic Details
Published in:J Biol Chem
Main Authors: Zhi, Xiaoduo, Dages, Samantha, Dages, Kelley, Liu, Yingting, Hua, Zi-Chun, Makemson, John, Leng, Fenfei
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2017
Subjects:
Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5582848/
https://ncbi.nlm.nih.gov/pubmed/28696257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.794628
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