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MONOLAYER-CRYSTAL STREPTAVIDIN SUPPORT FILMS PROVIDE AN INTERNAL STANDARD OF CRYO-EM IMAGE QUALITY

Analysis of images of biotinylated Escherichia coli 70S ribosome particles, bound to streptavidin affinity grids, demonstrates that the image-quality of particles can be predicted by the image-quality of the monolayer crystalline support film. The quality of the Thon rings is also a good predictor o...

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Detalles Bibliográficos
Publicado en:J Struct Biol
Main Authors: Han, Bong-Gyoon, Watson, Zoe, Cate, Jamie H. D., Glaeser, Robert M.
Formato: Artigo
Idioma:Inglês
Publicado: 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5581310/
https://ncbi.nlm.nih.gov/pubmed/28259651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2017.02.009
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